CHEM 240: Introduction to
Bioanalytical Chemistry

J. D. Cronk
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14. header

Lecture 14. Applications of spectrophotometry

Wednesday 16 February 2005

Applications of spectrophotometry. Determination of the concentrations of two unknowns using measurements at two wavelengths. Generalized problem of solving a system of two linear equations in two unknowns.

Reading: Harris - Ch.20, p.427-436.

 

14. Summary

Lecture 14 summary

The use of absorbance measurements at two wavelengths to determine the concentrations of two components of a solution can be treated as an example of a more generalized problem of solving a system of two linear equations in two unknowns. The term "linear" refers to the fact that only constants and terms in x and y (the two unknowns) appear in the equations, and there are no terms containing x2, xy, y2, norare there any other higher power polynomic terms. The application of a mathematical appraoch called matrix algebra allows a general solution to the problem of two equations in two unknowns. Note that in the equations in Harris on p.405 are just a specific instance of this general problem.

 
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